Intermediate

Part:BBa_K3440016:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


Mtrb under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part can be used to show that Mtrb can be expressed under constitutive promoter in Shewanella oneidensis mtrb knockout. In our project, we planned to use it as intermediate to build BBa_K3440019 and BBa_K3440021.

Source

Shewanella oneidensis

References